Antimicrobial Activity of Ganoderma lucidum Ethanol Extracts using Bioautography Method against Escherichia coli and Bacillus subtillis
Potensi Antimikroba Ekstrak Ethanol Ganoderma lucidum Menggunakan Metode Bioautografi terhadap Bakteri Escherichia coli dan Bacillus subtillis
AbstractThis study was aimed to determine the antibacterial potency of ethanol extract of lingzhi mushroom(Ganoderma lucidum) using bioautographic TLC method against Escherichia coli and Bacillus subtillis.Soxhletation method was chosen to extract the bioactive compound using the ethanol solvent. Following theextraction, the obtained extract was diluted to make a serial concentration which are 2%, 4%, 6%, 8% and10% with five replication each. Bioautographic TLC used n-butanol: glacial acetic acid: water (4: 1: 5) as adeveloping solvent. After complete development, the TLC plate was dried and observed for the spots whichare yellow spot with Retention factor (Rf) value of 0.3, blue spot with Rf 0.87, and brown spot with Rf 0.93.Based on the reference Rf value, this third detected spot is considered as triterpene compound. The spot wasthen tested for the inhibition zone. In the E. coli culture medium, the average value of the inhibition zone was5.45 mm (weak category) for the concentration of 2%, 5.14 mm for the 4%, 5.48 mm for the 6%, 5.94 mm forthe 8%, and 6.98 mm (medium category) for the 10%. Meanwhile in the Bacillus subtillis culture medium, aconcentration of 2% yielded 5.9 mm, 4% yielded 6.34 mm, 6% yielded 6.68 mm, 8% yielded 7.88 mm, and10% yielded 8.6 mm (medium category). In conclusion, the lingzhi mushroom ethanol extract hasantibacterial activity against both Escherichia coli and Bacillus subtillis with concentration-dependentmanner.
Copyright (c) 2021 Tri Puji Lestari Sudarwati, M.A. Hanny Ferry Fernanda
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