Effects of Reduction of MHCII Expression on M1 Polarity Activated HMC3 Microglia Cells By n-Hexane And Ethyl Acetate Fraction of Semanggi (Marsilea crenata Presl.) Leaves

Efek Penurunan Ekspresi MHCII Pada Sel Mikroglia HMC3 Teraktivasi M1 Polarity Oleh Fraksi n-Heksana Dan Etil Asetat Daun Semanggi (Marsilea crenata Presl.)

  • Burhan Ma'arif Program Studi Farmasi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Islam Negeri Maulana Malik Ibrahim, Malang
  • Faisal Akhmal Muslikh Mahasiswa Magister Ilmu Farmasi, Universitas Airlangga, Surabaya
  • Abdul Malik Guhir Program Studi Farmasi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Islam Negeri Maulana Malik Ibrahim, Malang
  • Hilwa Fitri Program Studi Farmasi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Islam Negeri Maulana Malik Ibrahim, Malang
  • Luqman Alfani Najib Program Studi Farmasi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Islam Negeri Maulana Malik Ibrahim, Malang
  • Novyananda Salmasfattah Program Studi Farmasi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Islam Negeri Maulana Malik Ibrahim, Malang
  • Hening Laswati Departemen Ilmu Kedokteran Fisik dan Rehabilitasi, Fakultas Kedokteran, Universitas Airlangga, Surabaya
  • Mangestuti Agil Departemen Ilmu Farmasi, Fakultas Farmasi, Universitas Airlangga, Surabaya
Keywords: Fitoestrogen, Marsilea crenata Presl., MHC II, Sel Mikroglia HMC3

Abstract

Neuroinflammatory is a pathological condition in which neuronal cells experience progressive loss of function, one of which is due to estrogen deficiency. Phytoestrogens are compound that has structure and function similar to 17β-estradiol, the most potent natural form of estrogen. Semanggi (Marsilea crenata Presl.) is known to contain phytoestrogens which can replace the function of 17β-estradiol in maintaining organ homeostasis, so that is potential to be developed as an anti-neuroinflammatory drug. This study was aimed to prove the anti-neuroinflammatory effect of the n-hexane and ethyl acetate fraction of Semanggi leaves on HMC3 microglia cells, by measuring the expression of major histocompatibility complex II (MHC II). Both fractions with a concentration of 62.5; 125; and 250 μg/ml were administered to HMC3 microglia cells which had been induced by interferon gamma (IFN-γ) for 24 hours. Measurement of MHC II expression were using immunocytochemistry (ICC) methods and confocal laser scanning microscopy (CLSM) instruments. The results showed that n-hexane fraction could reduce MHC II expression at concentrations of 250 μg/ml with p<0.05, and showed a non-monotonic dose response (NMDR) pattern, while ethyl acetate fraction could reduce MHC II expression at all concentrations with p<0.05. Based on the results of the correlation test, the best anti-neuroinflammatory activity on HMC3 microglia cells is showed by the ethyl acetate fraction of Semanggi leaves.
Published
2022-02-01
How to Cite
Ma’arif, B., Akhmal Muslikh, F., Malik Guhir, A., Fitri, H., Alfani Najib, L., Salmasfattah, N., Laswati, H., & Agil, M. (2022). Effects of Reduction of MHCII Expression on M1 Polarity Activated HMC3 Microglia Cells By n-Hexane And Ethyl Acetate Fraction of Semanggi (Marsilea crenata Presl.) Leaves: Efek Penurunan Ekspresi MHCII Pada Sel Mikroglia HMC3 Teraktivasi M1 Polarity Oleh Fraksi n-Heksana Dan Etil Asetat Daun Semanggi (Marsilea crenata Presl.). Journal Pharmasci (Journal of Pharmacy and Science), 7(1), 35-41. https://doi.org/10.53342/pharmasci.v7i1.271