Antioxidant Activity Test of Ubiquinone Microemulsion with DPPH (1,1-Diphenyl-2-picrylhydrazyl) Method

Uji Aktivitas Antioksidan Mikroemulsi Ubiquinone dengan Metode DPPH (1,1-Difenil-2-pikrilhidrazil)

  • Silvi Ayu Wulansari Akademi Farmasi Surabaya
  • Umarudin Akademi Farmasi Surabaya
  • Floreta Fiska Yuliarni Akademi Farmasi Surabaya
Keywords: Ubiquinone, Antioxidant, Microemulsion, DPPH

Abstract

Ubiquinone is an antioxidant formulated in cosmetic preparations. It is necessary to test the atioxidant activity to ensure the quality of the preparation. The purpose of this study was to determine the antioxidant activity of the ubiquinone formulated in microemulsion preparations. The Ubiquinone were formulated into mikroemultion with cocentrations 0.25%, 0.5%, 0.75% and 1%, separately. The DPPH method was selected as the atioxidant activity test.. The data analysis in this study was descriptive to determine the IC50 value of ubiquinone microemulsion preparations F1, F2, F3 and F4 and ubiquinone as a comparison. The result shows that the antioxidant value of IC50 ubiquinone microemulsion of F1, F2, F3, and F4 are 56.48 ppm, 49.92 ppm, 32.76 ppm, and 20.71 ppm, respectively. The average value of ubiquinone microemulsion is 59.16 ppm. This research concludes that the microemulsion formula F1-F4 of ubiquinon has a very strong antioxidant activity compared to ubiquinone.

References

1. Ardhie A. Radikal Bebas dan Peran Antioksidan dalam Mencegah Penuaan. Medicinus Anti Aging. Sci J Pharm Dev Med Appl. 2011
2. Montenegro L. Nanocarriers for skin delivery of cosmetic antioxidants. J Pharm Pharmacogn Res. 2014;2(4):73–9
3. Eman, S., Makky, A.M., Khattab A.M., et al. Nanoemulsion Gel of Neutraceutical Co_Enzym Q10 As An Alternative To Convetional Topical Delivery System To Enhancer Skin Permeability And Anti Wrinkle Efficiency. International Journal of Pharmaceutical Sciences, 2017. Vol. 9 issue 11, p.208-214
4. Lucangioli, S., Tripodi, V.The Importance Of The Formulation In The Effectiveness Of Coenzyme Q10 Supplementation In Mitochondrial Disease Therapy, Der Pharmacia Sinica. 2012;3(4), 406-407.
5. Deapsari, Fani. Penetration of Ubiquinone (Q10) Nanoemulsion Using Olive Oil Through Rat Skin, International Journal of Pharmaceitical and clinical Research.2016;9(2), 169-172.
6. Mawarni, F. Formulasi Krim Ekstrak Etanol Daun Salam (Syzygium Polyanthum (Wight) Walp.) dan Uji Aktivitas Antioksidan Dengan Metode DPPH. Skripsi. Universitas Setia Budi; 2018
7. Purwanto, D. Bahri, S., dan Ridhay, A. Uji Aktivitas Antioksidan Ekstrak Buah Purnajiwa (Kopsia Arborea Blume.) Dengan Berbagai Pelarut. Kovalen. 2017;3(1): 24 – 32
8. Jun M, Fu HY, Hong J, Wang X,Yang CS, Ho CT. Comparison of antioxidant activities of isoflavones from kudzu root (Pueraria lobate ohwi). J of Food Science. 2006;2117-22
9. Maesaroh, K., Kurnia, D., Al Anshori, J. Perbandingan Metode Uji Aktivitas Antioksidan DPPH, FRAP dan FIC Terhadap Asam Askorbat, Asam Galat dan Kuersetin, Jurnal Chimica et Natura Acta. 2018;6 (2).
10. Mishra, A. K., Mishra, A., Chattopadhyay, P. Formulation and In-Vitro Evaluation of Antioxidant Activity of O/W Sunscreen Cream Containing Herbal Oil as Dispersed Phase. International Journal of Biomedical Research.2010; 5: 201-208.
11. Wulansari SA, Lubada EI. Validasi Metode Analisa Pada Penetapan Kadar Koenzim Q10 Dalam Sediaan Mikroemulsi Menggunakan Metode Spektrophotometer Uv-Vis. J Kim Ris. 2020;5(1):29
12. Molyneux P.The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating anti-oxidant activity. Songklanakarin Journal of Science and Technology. 2004;26(May), 211–219.
13. Rizkayanti, Diah, A. W. M., & Jura, M. R. Uji Aktivitas Antioksidan Ekstrak Air dan Ekstrak Etanol Daun Kelor (Moringa Oleifera LAM). Jurnal Akademika Kimia. 2017;6(2), 125–131
14. Rohman,A. Validasi Kimia. Yogyakarta: Gajah Mada Univeritas Press.dan Penjaminan Mutu Metode Analisis; 2016
15. Setiawan, F., Yunita, O., Kurniawan, A. Uji Aktivitas Antioksidan Ekstrak Etanol Kayu Secang (Caesalpinia sappan) Menggunakan Metode DPPH, ABTS, dan FRAP, Artikel Penelitian, Fakultas Farmasi Universitas Surabaya; 2018
Published
2022-08-03
How to Cite
Wulansari, S. A., Umarudin, & Yuliarni, F. F. (2022). Antioxidant Activity Test of Ubiquinone Microemulsion with DPPH (1,1-Diphenyl-2-picrylhydrazyl) Method : Uji Aktivitas Antioksidan Mikroemulsi Ubiquinone dengan Metode DPPH (1,1-Difenil-2-pikrilhidrazil). Journal Pharmasci (Journal of Pharmacy and Science), 7(2), 125-130. Retrieved from http://ejournal.akfarsurabaya.ac.id:80/index.php/jps/article/view/286