Phytochemical Screening of Chloroform Extract from Javanese Long Pepper which Dried Through 2 Different Methods

Skrining Fitokimia Ekstrak Kloroform dari Buah Cabe Jawa yang Dikeringkan dengan 2 Metode Berbeda

  • Diah Wulan Safitri Akademi Farmasi Surabaya
  • Meyke Herina Syafitri Akademi Farmasi Surabaya
Keywords: Blanching, Phytochemical, Chloroform, Drying, Piper retrofractum

Abstract

Fruit is easily damaged and has a short shelf life due to the high water content. Drying is one of the post-harvest handling processes that can reduce the water content of the product to increase stability during product distribution and storage. Javanese long pepper (Piper retrofractum Vahl.) is included in the 10 largest raw materials absorbed by the traditional medicine industry. Purpose of this study is to carry out phytochemical screening of chloroform extract from javanese long pepper which dried through 2 different methods. Group 1 was air-dried, while group II was pre-treated with blanching, which was boiled for a few minutes before drying in direct sunlight. The dried implicia produced from both groups were macerated with chloroform for 3 days each. The maceration filtrate then tested to determine the secondary metabolite compounds contained in it through phytochemical screening. The test is carried out by observing the presence or absence of a precipitate formed or the color change that occurs after the addition of reagents. The results showed that both groups were positive for alkaloids, flavonoids and steroids, but negative for terpenoids, tannins, and saponins. There was no difference in content between the two treatment groups.

References

1. Gita RSD, Danuji S. Studi Keanekaragaman Tumbuhan Obat Yang Digunakan Dalam Pengobatan Tradisional Masyarakat Kabupaten Pamekasan. Bioma J Biol dan Pembelajaran Biol. 2021;6(1):11–23.
2. Yosika NIW, Hawa LC, Hendrawan Y. Characteristics and drying rate of cabya (Piper retrofractum Vahl.) with natural drying method (open sun drying). J Teknol Pertan. 2020;21(3):165–74.
3. Di V, Sentra B, Penelitian B, Obat T. Karakteristik Morfologi Tanaman Cabe Jawa (Piper retrofractum. Vahl) Di Beberapa Sentra Produksi. Bul Penelit Tanam Rempah dan Obat. 2015;20(1):1–10.
4. Mutu A, Dan F, Jawa K, Metode D, Oven P, Dan K. Analisi Mutu Fisik Dan Kimia Cabai Jawa (Piper refractum Vahl) Dengan Metode Pengeringan Oven Kabinet Dan Dan Pengeringan Sinar Matahari. J Teknol Ind Pertan. 2021;15(4):1001–10.
5. Cho SN, Chatterjee D, Brennan PJ. Skrining Fitokimia Dan Kandungan Total Flavonoid Pada Buah Carica pubescens Lenne & K. Koch Di Kawasan Bromo, Cangar, Dan Dataran Tinggi Dieng. Am J Trop Med Hyg. 1986;35(1):167–72.

6. Courtney A. Farmakope Herbal Indonesia Edisi II. Kementerian Kesehatan Republik Indonesia. 2017. 1–534 p.
7. Ari Parfiyanti E, Budihastuti R, Dwi Hastuti E, Biologi J, Sains dan Matematika F. Pengaruh Suhu Pengeringan Yang Berbeda Terhadap Kualitas Cabai Rawit (Capsicum frutescens L.). J Biol. 2016;5(1):82–92.
8. Lestari N, Fadilah R, Mukhlis AMA, Samsuar S. Efek Perlakuan Low Temperature Long Time Blanching Terhadap Karakteristik Cabai Kering. Agrika. 2020;14(2):140.
9. Sofiyanti N. Skrining Fitokimia Lima Jenis Tumbuhan Paku Polypodiaceae Dari Provinsi Riau Phytochemical Screening of Five Species of Polypodiaceae Ferns from Riau Province. Biota. 2019;4(2):40–9.
10. Parbuntari H, Prestica Y, Gunawan R, Nurman MN, Adella F. Preliminary Phytochemical Screening (Qualitative Analysis) of Cacao Leaves (Theobroma cacao L.). EKSAKTA. 2018;19(2):40–5.
11. Makhawi AM, Hamadnalla H. Phytochemical Screening of Leaves and Roots of Stylochiton Borumensis: A Medicinal Plant. Earth Environ Sci Res Rev. 2019;2(1):1–5.
12. Gul R, Jan SU, Faridullah S, Sherani S, Jahan N. Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan. Sci World J. 2017;2017(Figure 1).
13. Dewatisari WF. Perbandingan Pelarut Kloroform dan Etanol terhadap Rendemen Ekstrak Daun Lidah Mertua ( Sansevieria trifasciata Prain .) Menggunakan Metode Maserasi. JournalUin-Alauddin [Internet]. 2020;(September):127–32. Available from: http://journal.uin-alauddin.ac.id/index.php/psb/
14. Wardana AP, Tukiran. Skrining Fitokimia Dan Aktivitas Antioksidan Ekstrak Kloroform Tumbuhan Gowok ( Syzygium polycephalum ). Pros Semin Nas Kim. 2016;(September):1–6.
15. Muthmainnah. Skrinning Fitokimia Senyawa Metabolit Sekunder Ekstrak Etanol Buah Delima (Punica granatum L.) Dengan Metode Uji Warna. Media Farm. 2017;13(2):23–8.
16. Tjitda PJP, Nitbani FO. Phytochemical Screening of Methanol , Chloroform and n-Hexane Extract from Flamboyant Leaves ( Delonix regia . Raf ) from Kupang. J Sains Dan Terap Kim. 2019;13(2):70–9.
17. Minarno EB. Skrinning Fitokimia Dan Kandungan Total Flavonoid Pada Buah Carica pubescens Lenne & K.Koch Di Kawasan Bromo, Cangar Dan Dataran Tinggi Dieng. El-Hayah. 2015;5(2):73–82.
18. Sulistyowati D. Pengaruh Intensitas Naungan Terhadap Pertumbuhan dan Kandungan Bioaktif Daun Dua Aksesi Tanaman Cabe Jawa (Piper retrofractum Vahl.). J Penyul Pertan. 2010;5(2):105–15.
19. Muslichah S. Potensi Afrodisiak Kandungan Aktif Buah Cabe Jawa (Piper retrofractum Vahl) pada Tikus Jantan Galur Wistar. J Agrotek. 2011;5(2):11–20.
20. Astar ina, N. W. G.1, Astuti, K. W.1, Warditiani NK. Skrining Fitokimia Ekstrak Metanol Rimpang Bangle (Zingber purpereum Roxb). J Farm Udayana. 2013;3(3):1–7.
21. Oktavia FD, Sutoyo S. Skrining Fitokimia, Kandungan Flavonoid Total, Dan Aktivitas Antioksidan Ekstrak Etanol Tumbuhan Selaginella doederleinii. J Kim Ris. 2021;6(2):141–53.
Published
2022-08-03
How to Cite
Safitri, D. W., & Syafitri, M. H. (2022). Phytochemical Screening of Chloroform Extract from Javanese Long Pepper which Dried Through 2 Different Methods: Skrining Fitokimia Ekstrak Kloroform dari Buah Cabe Jawa yang Dikeringkan dengan 2 Metode Berbeda. Journal Pharmasci (Journal of Pharmacy and Science), 7(2), 137-142. https://doi.org/10.53342/pharmasci.v7i2.292