Antibacterial Assay Of Cyperus rotundus Rhizomes Against Gram Positive And Gram Negative Bacteria

Uji Potensial Antibakteri Umbi Rumput Teki (Cyperus rotundus) terhadap bakteri Gram positif dan bakteri Gram negatif

  • Diana Yuni Anjarsari Akademi Farmasi Surabaya
  • Kinanti Ayu Puji Lestari Akademi Farmasi Surabaya
Keywords: Nut grass tuber antibacterial, Nut grass tuber, Gram positive bacteria, Gram negative bacteria

Abstract

Studies on antibacterial tests derived from natural ingredients were more intensively carried out. This was to reduce the side effects of using antibiotics related to the incidence of pathogenic bacteria. Nut grass tubers were one of the natural ingredients that are widely known for their benefits for human health. Nut grass tubers were also known to contain antibacterial compounds such as flavonoids, tannins, saponins and so on. This study aims to determine the potential of nut grass tuber extract as antibacterial of Gram positive and Gram negative. This study used a completely randomized design with the treatment of teki grass tuber extract with concentrations of 25%, 50% and 100%. Based on the research, it was known that the nut grass tubers were able to inhibit the growth of Gram positive and Gram negative bacteria in the category of weak to moderate ability. This study used the treatment of teki grass tuber extract with concentrations of 25%, 50% and 100%. Based on the research, it was found that the tuber of teki grass was able to inhibit the growth of Gram positive bacteria with the highest inhibition at 100% concentration of 5.53 mm and Gram negative with the highest inhibition at 100% concentration of 6.57 mm.

References

1. Hu, QP., Cao, XM.,Hao, DL., Zhang, LL. Chemical Composition, Antioxidant, DNA Damage Protective, Cytotoxic and Antibacterial Activities of Cyperus rotundus Rhizomes Essential Oil against Foodborne Pathogen. Scientific Report. 7:45231. www.nature.com/scientificreport. DOI: 10.1038/srep45231. 2017
2. Hemanth Kumar, K., Razack, S., Nallamuthu, I. & Khanum, F. Phytochemical analysis and biological properties of Cyperus rotundus L. Ind. Crop Prod. 52, 815–826. 2014.
3. Peerzada, A. M., Ali, H. H., Naeem, M., Latif, M., Bukhari, A. H. & Tanveer, A. Cyperus rotundus L.: Traditional uses, phytochemistry, and pharmacological activities. J. Ethnopharmacol. 174, 540–560. 2015.
4. Kilani, S. et al. In vitro evaluation of antibacterial, antioxidant, cytotoxic and apoptotic activities of the tubers infusion and extracts of Cyperus rotundus. Bioresource Technol. 99, 9004–9008 2008.
5. Kilani-Jaziri, S., Bhouri, W., Skandrani, I., Limem, I., Chekir-Ghedira, L. & Ghedira, K. Phytochemical, antimicrobial, antioxidant and antigenotoxic potentials of Cyperus rotundus extracts. S. Afr. J. Bot. 77, 767–776. 2011.
6. Badgujar, S. B. & Bandivdekar, A. H. Evaluation of a lactogenic activity of an aqueous extract of Cyperus rotundus Linn. J. Ethnopharmacol. 163, 39–42. 2015.
7. Xu, H.-B. et al. Bioactivity-guided isolation of anti-hepatitis B virus active sesquiterpenoids from the traditional Chinese medicine: Rhizomes of Cyperus rotundus. J. Ethnopharmacol. 17, 1131–140. 2015.
8. Nam, J. H., Nam, D.-Y. & Lee, D.-U. Valencene from the rhizomes of Cyperus rotundus inhibits skin photoaging-related ion channels and UV-induced melanogenesis in B16F10 melanoma Cells. J. Nat. Prod. 79, 1091–1096. 2016.
9. Lamaga FE, Nazila H, Fitri R, Marwati F, Putri S. Uji Sifat Fisik dan Aktivitas Antibakteri Krim Ekstrak Etanol Umbi Rumput TekiI Terhadap Staphylooccus aureus Physical Properties And Antibacterial Activity of Cream from Ethanol Extracts Of Nut Grass Against Staphylococcus aureus. J Kesehat Madani Med. 2019;10(1):20–6.
10. Tiara Dewi, Muhammad Amir Masruhim RS. Lab Penelit dan Pengemb Farmaka Trop Fak Farm Univ Mualawarman, Samarinda, Kalimantan Timur. 2016;04(April):5–24.
11. Niazta NA, Hermanto B, Wasito EB. Daya Antibakteri Infusa Umbi Rumput Teki terhadap Escherichia coli Secara In Vitro. Juxta J Ilm Mhs Kedokt Univ Airlangga. 2015;7(1):1–7.
12. Made N, Soegianto L, Hermanu LS. Uji Potensi Antibakteri dan Antibiofilm Minyak Atsiri Umbi Teki ( Cyperus rotundus L .) terhadap Staphylococcus aureus ATCC. Pharm Sci Pract. 2017;4(2):76–81.
13. Sari L, Lesmana D, Taharuddin. Estraksi minyak atsiri dari daging buah pala (tinjauan pengaruh metode destilasi dan kadar air bahan ). Semin Nas Sains dan Teknol 2018. 2018;919:1–6.
14. Buana Januarti I, Santoso A, Razak AS. Flavonoid Extraction of Teak Leaf (Tectona grandis L.) with Ultrasonic Method (Study Of Material:Solvent Ratio and Extraction Time) Program Studi Farmasi Fakultas Kedokteran Universitas Islam Sultan Agung Jl. Kaligawe KM 4 Semarang 50012 Telp.(+6224) 6583. 12(2):1263–70.
15. Sa’adah H, Nurhasnawati H. Perbandingan Pelarut Etanol Dan Air Pada Pembuatan Ekstrak Umbi Bawang Tiwai (Eleutherine americana Merr) Menggunakan Metode Maserasi. J Ilm Manuntung. 2017;1(2):149.
16. Widyaningrum KS, Setiawan DK, Kaloko BS. Pengaruh Variasi Suhu Destilasi Terhadap Karakteristik Minyak Jarak Sebagai Alternatif Isolasi Cair pada Transformator Daya. Berk Sainstek. 2017;5(1):41.
17. Teki R, Analisis LD. Identifacation Of Chemical Compound In Nut Grass ( Cyperus rotundus L .) Tuber N-Hexane Extract By GC-MS Analysis Identifikasi Komponen Senyawa Dalam Ekstrak N-Heksana Umbi. 2021;10:975–84.
18. Yunikawati MPA, Besung INK, Mahatmi H. Efektifitas Perasan Daun Srikaya Terhadap Daya Hambat Pertumbuhan Escherichia coli. Ef Perasan Daun Srikaya Terhadap Daya Hambat Pertumbuhan Escherichia coli [Internet]. 2013;2(2):170–9. Available from: http://ojs.unud.ac.id/index.php/imv/article/view/5525
19. Putri Ningsih A, dan Anthoni Agustien N. Uji Aktivitas Antibakteri Ekstrak Kental Tanaman Pisang Kepok Kuning (Musa paradisiaca Linn.) terhadap Staphylococcus aureus dan Escherichia coli Antibacterial Activity of Crude Extracts of Pisang Kepok Kuning (Musa paradisiaca Linn.) Against staphylococc. J Biol Univ Andalas (J Bio UA). 2013;2(3):207–13.
20. Mulyadi M, Wuryanti W, Sarjono PR. Konsentrasi Hambat Minimum (KHM) Kadar Sampel Alang-Alang (Imperata cylindrica) dalam Etanol Melalui Metode Difusi Cakram. J Kim Sains dan Apl. 2017;20(3):130–5.
Published
2022-08-01
How to Cite
Anjarsari, D. Y., & Lestari, K. A. P. (2022). Antibacterial Assay Of Cyperus rotundus Rhizomes Against Gram Positive And Gram Negative Bacteria: Uji Potensial Antibakteri Umbi Rumput Teki (Cyperus rotundus) terhadap bakteri Gram positif dan bakteri Gram negatif. Journal Pharmasci (Journal of Pharmacy and Science), 7(2), 95-99. https://doi.org/10.53342/pharmasci.v7i2.294