Kinetic Effects of Temperature and Storage Time on Vitamin C Levels in Lemon Juice

Studi Kinetika Pengaruh Suhu dan Lama Waktu Penyimpanan terhadap Kadar Vitamin C dalam Jus Lemon

  • Winona Ammadea Gloryani Tapikap Akademi Farmasi Surabaya
  • Vika Ayu Devianti Akademi Farmasi Surabaya
Keywords: Vitamin C, Lemon Juice, Storage Temperature, Storage Kime, Kinetics

Abstract

Lemon (Citrus limon L.) is one of the fruits with the highest vitamin C content. Lemon (Citrus limon L.) is well-known as an ingredient for squeezing and extracting its juice for making beverages. In the process, the vitamin C contained in lemon juice (Citrus limon L.) is easily lost due to oxidation during processing and storage. Therefore, this study aims to determine the effect of storage temperature on vitamin C levels of lemon juice (Citrus limon L.) using UV-Vis spectrophotometry method. This research was conducted experimentally with variations in room temperature and cold storage temperature for 24 hours. The absorbance measurement of the sample was carried out at a wavelength of 265.5 nm. The results showed that variations in storage temperature had an effect on vitamin C levels in lemon juice (Citrus limon L.). The significant value of the F-test was 0.001 and the significant value of the T test at temperature and storage time were 0.02 and 0.000, respectively. The decrease in vitamin C levels followed a zero order kinetic model.  Vitamin C levels at cold temperature were lower than room temperature as indicated by the value of the reaction rate constant at cold temperature which was lower than room temperature.

Author Biography

Vika Ayu Devianti, Akademi Farmasi Surabaya
Pharmacy

References

[1]. Trisnawati I, Hersoelistyorini W, Nurhidajah N. Tingkat Kekeruhan Kadar Vitamin C dan Aktivitas Antioksidan Infused Water Lemon Dengan Variasi Suhu Dan Lama Perendaman. J Pangan dan Gizi. 2019;9(1):27.
[2]. Babashahi-Kouhanestani M, Salehi M, Mazloomi S, Almasi-Hashyani A. Quantitative evaluation of vitamin C in industrial lemon juice by titration method. J Biol Today’s World. 2014;3(6):139–41.
[3]. Mushtaq MW, Tariq S, Hameed A, Bashir S, Bibi S, Dogar NA, et al. Spectrophotometric Determination of Vitamin C In Underground Vegetables and Kinetic Modelling to Probe The Effect of Temperature And pH on Degradation Of Vitamin C. Pakistan J Bot. 2022;54(5):1771–5.
[4]. El-Ishaq A, Obirinakem S. Effect of Temperature and Storage on Vitamin C Content in Fruits Juice. Int J Chem Biomol Sci. 2015;1(2):17–21.
[5]. Fauziah. Pengaruh Suhu Penyimpanan dan Jenis Kemasan Serta Lama Penyimpanan Terhadap Karakteristik Tomat (Solanum lycopersicum L.) Organik. J Teknol Pangan. 2010;11(30):1–42.
[6]. Kaleem A, Nazir H, Pervaiz S, Iqtedar M, Abdullah R, Aftab M, et al. Investigation of the effect of temperatute on vitamin C in fresh and packed fruit juices. FUUAST J Biol. 2016;6(1):117–20.
[7]. Asgar A. Effect of storage temperature and type of packaging on physical and chemical quality of carrot. IOP Conf Ser Earth Environ Sci. 2020;443(1).
[8]. Oyetade, O.A., Oyeleke, G.O., Adegoke, B.M. and Akintunde AO. Stability Studies on Ascorbic Acid (Vitamin C) From Different Sources. IOSR J Appl Chem. 2012;2(4):20–4.
[9]. Sapei L, Hwa L. Study on the Kinetics of Vitamin C Degradation in Fresh Strawberry Juices. Procedia Chem. 2014;9:62–8.
[10]. Asmara AP, Amungkasi HK. Kajian Kinetika Pengaruh Lama Penyimpanan terhadap Kadar Vitamin C pada Buah Apel Malang (Malus sylvestris). Al-Kimia. 2020;7(2):136-146.
[11]. Husnun F, Daryono BS, Fitriani A, Supriyadi S. Sifat Kimia dan Kinetika Degradasi Termal Antioksidan Jus Melon (Cucumis melo L.) Kultivar Gama Melon Parfum. J Teknol Pertan Andalas. 2022;26(1):71–83.
[12]. Swadana AW, Yuwono SS. Pendugaan Umur Simpan Minuman Berperisa Apel menggunakan Metode Accelerated Shelf Life Testing (ASLT) dengan Pendekatan Arrhenius. J Pangan dan Agroindustri. 2014;2(3):203–12.
[13]. Amanto BS, Ishartani D, Nurulaini A. Kinetika Degradasi L-Asam Askorbat Pada Proses Pasteurisasi Puree Jambu Biji (Psidium guajava) Varietas Getas Merah. J Teknol Has Pertan. 2016;IX(1):62–70.
[14]. Watson GD. Pharmaceutical Analysis: A textbook for pharmacy students and pharmaceutical chemists. 2005. 49–71.
[15]. Damayanti ET, Kurniawati P. Perbandingan Metode Penentuan Vitamin C pada Minuman Kemasan Menggunakan Metode Spektrofotometer UV-Vis dan Iodimetri. Pros Semin Nasoinal Kim dan Pembelajarannya. 2017;(November):258–67.
[16]. Blongkod NA, Wenur F, Longdong IA. Kajian Pengaruh Pra Pendinginan Dan Suhu Penyimpanan Terhadap Umur Simpan Brokoli. Cocos. 2016;7(5).
[17]. Kumar N, Kachhadiya S, Nayi P. Storage stability and characterization of biochemical , rehydration and colour characteristics of dehydrated sweet corn kernels. J Stored Prod Res. 2020;87:101619.
[18]. Tambunan IJ, Sulasmi S, Julianty SM. Kajian Kinetika Penentuan Laju Reaksi Penetapan Kadar Vitamin C Pada Brokoli (Brassica oleracea L) Dengan Metode Titrasi Titrimetri. J Pharm Sci. 2023;6(1):1–7.
[19]. Rahman MS, Al-Rizeiqi MH, Guizani N, Al-Ruzaiqi MS, Al-Aamri AH, Zainab S. Stability of vitamin C in fresh and freeze-dried capsicum stored at different temperatures. J Food Sci Technol. 2015;52(3):1691–7.
[20]. Khatir R, Ratna R, Puri MA. Pendugaan Umur Simpan Jagung Manis Berdasarkan Kandungan Total Padatan Terlarut Dengan Model Arrhenius. J Agritech. 2015;35(02):200.
Published
2023-07-19
How to Cite
Ammadea Gloryani Tapikap, W., & Devianti, V. A. (2023). Kinetic Effects of Temperature and Storage Time on Vitamin C Levels in Lemon Juice : Studi Kinetika Pengaruh Suhu dan Lama Waktu Penyimpanan terhadap Kadar Vitamin C dalam Jus Lemon. Journal Pharmasci (Journal of Pharmacy and Science), 8(2), 119-125. https://doi.org/10.53342/pharmasci.v8i2.365