Antibacterial Activity Test of Sargassum polycystum in Staphylococcus aureus

Uji Aktivitas Antibakteri Sargassum polycystum terhadap Bakteri Staphylococcus aureus

Abstract

The objective of this study was to evaluate the antibacterial activity of brown seaweed Sargassum
polycystum ethanol extract 96% to Staphylococcus aureus bacteria. S. polycystum is coming from Cabbiya
village, Talango sub-district, Sumenep district, Madura that is extracted with ethanol 96% with maceration
method. This research used five treatments with three repetitions. Each treatment consist of brown
seaweed S.polycystum ethanol extract 96% in some concentrations, they were 10% w/v, 20% w/v, 40% w/v, 80%
w/v, and 100% w/v, positive control used Chloramphenicol 0,1%, and negative control used ethanol extract 96%
that was used for extract solvent. Antibacterial test of brown seaweed S.polycystum ethanol extract 96%
used well diffusion method and the number of bacteria was adjusted to Mc Farland turbidity standard.After
collecting data The data that was obtained was analyzed using One-way ANOVA in SPSS statistical application.
The result showed that brown seaweed S.polycystum ethanol extract 96% had the antibacterial activity
to S.aureus. The measuring of inhibition zone results were: in 10% concentration was 3,49 ± 3,55 mm; 20%
concentration was 4,22 ± 3,91 mm; 40% concentration was 5,97 ± 5,09 mm; 80% concentration was 8,41 ± 2,76
mm; and 100% concentration was 11,07 ± 0,07 mm.