Effect of Aqueous Extract of Kunyit Hitam (Curcuma caesia Roxb.) Using Green Solvent Method on Mice Blood Glucose (Mus musculus)

Pengaruh Pemberian Ekstrak Air Kunyit Hitam (Curcuma caesia Roxb.) Dengan Metode Green Solvent Terhadap Kadar Glukosa Darah Mencit (Mus musculus)

Abstract

Black turmeric contains flavonoids which can suppress the decline in blood glucose by restoring sensitivity to insulin secreted by pancreatic beta cells. Apart from that, flavonoids can reduce blood glucose levels by several mechanisms. This study aims to determine the effect of administering black turmeric (Curcuma caesia roxb.) water extract using the green solvent method on the blood glucose levels of mice (Mus musculus L). The research method uses a green solvent-based Microwave-Assisted Extraction (MAE) extraction method with the solvent used being choline chloride-sorbitol. Thirty male mice were divided into 6 treatment groups, namely negative group, test I (dose 25 mg/kgBB), test II (dose 50 mg/kgBB), test III (dose 100 mg/kgBB), test IV (dose 200 mg /kgBB) and V test (dose 400 mg/kgBB). Mice were induced with alloxan monohydrate at a dose of 210 mg/kgBB intraperitoneally for 3 days. hen black turmeric water extract was given for 7 days and blood glucose levels were checked on the 8th day. Based on the research results, it was concluded that the mean decrease value in the negative group had increased by 47.6 mg/dl, test I (dose 25 mg/kgBB) had increased by 5 mg/dl, test II (dose 50 mg/kgBB) had an increase of 9 mg/dl, test III (dose 100 mg/kgBW) experienced a decrease of 43 mg/dl, test IV (dose 200 mg/kgBW) experienced a decrease of 139.3 mg/dl and test V (dose 400 mg/dl kgBB) had the highest glucose level reduction activity with an average reduction of 182.3 mg/dl compared to the other test groups.